Contents

          1. Genomic DNA Isolation

          2. RNA Isolation

          3. PCR(Polymerase Chain Reaction)

          4. PAGE

          5. QIAquick PCR purification

          6. Gene Cloning

              (1) khs T vector

              (2) Agarose Gel Purification

              (3) Ligation

            (4) Transformation I/II   

            (5) Inoculation

            (6) Plasmid DNA Isolation

          7. DNA Sequencing

          8. Phage Amplification & cDNA library Isolation

          9. Southern & Northern Blotting

  10. Data Analysis

(1)   Comparative genomics

(2)   MEboot Analysis Manual

(3)   MEGA2 Analysis Manual